Anti-Cytokeratin, Multi (Epithelial Marker) Monoclonal Antibody(Clone: KRT/1877R)
Fig. 1: Formalin-fixed, paraffin-embedded human Skin stained with Multi-Cytokeratin Recombinant Rabbit Monoclonal Antibody (KRT/1877R).
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| Amount : | 100 µg |
| Isotype : | Rabbit IgG |
| Content : | 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage condition : | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. |
| Gene ID : | 3851; 3852; 3853; 3854; 286887; 3856; 3858; 3860; 3875 |
| Uniprot ID : | P02538 ; P04259 ; P13647 ; P19013 ; P48668 |
| Immunogen Information : | Keratin-enriched preparation from cultured human epithelial cells |
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13) and 45kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Flow Cytometry (0.5-1µg/million cells); Immunofluorescence (0.5-1.0µg/ml); Western Blot (0.5-1.0µg/ml); Immunohistochemistry (Formalin-fixed) (0.5-1.0µg/ml for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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