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  7. Anti-E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Monoclonal Antibody(Clone: 4A2)

Anti-E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Monoclonal Antibody(Clone: 4A2)

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Fig. 1: Formalin-fixed, paraffin-embedded human Skin stained with E-Cadherin Mouse Monoclonal Antibody (4A2).

Anti-E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Monoclonal Antibody(Clone: 4A2)
Anti-E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Monoclonal Antibody(Clone: 4A2)
Anti-E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Monoclonal Antibody(Clone: 4A2)
Anti-E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Monoclonal Antibody(Clone: 4A2)
Anti-E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Monoclonal Antibody(Clone: 4A2)

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Fig. 1: Formalin-fixed, paraffin-embedded human Skin stained with E-Cadherin Mouse Monoclonal Antibody (4A2).
Fig. 2: Western Blot Analysis of MCF-7 cell lysate using E-Cadherin Mouse Monoclonal Antibody (4A2).
Fig. 3: Immunofluorescence Analysis of MCF-7 cells labeling E-Cadherin with E-Cadherin Mouse Monoclonal Antibody (4A2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)
Fig. 4: Flow Cytometric Analysis of human trypsinized MCF-7 cells using E-Cadherin Mouse Monoclonal Antibody (4A2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
Fig. 5: SDS-PAGE Analysis Purified E-Cadherin Mouse Monoclonal Antibody (4A2). Confirmation of Integrity and Purity of Antibody.

Product code: 36-3677

Clone name : 4A2
Clonality : Monoclonal
Application : FACS, IF, WB, IHC
Reactivity : Human

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  •   20 µg

  •  100 µg

  • $199.00 

  • $459.00 

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  • Product Info
  • Antigen Details
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Amount : 100 µg
Isotype : Mouse IgG1, kappa
Content : 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Storage condition : Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
Gene : CDH1
Gene ID : 999
Uniprot ID : P12830
Alternative Name : Arc 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD324; CDH1; CDHE; E-Cad/CTF3; E-cadherin; ECAD; Epithelial cadherin; epithelial calcium dependent adhesion protein; Liver cell adhesion molecule (LCAM); Uvomorulin (UVO)
Immunogen Information : Recombinant human E-Cadherin protein
Recognizes a protein of 120-80kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as -catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.

Flow Cytometry (1-2µg/million cells); Immunofluorescence (1-4µg/ml); ,Western Blot (0.5-1µg/ml); ,Immunohistochemistry (Formalin-fixed) (0.5-1µg/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Monoclonal Antibody(Clone: 4A2)

Product code: 36-3677
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