Rabbit Polyclonal Antibody to TAP-tag(Discontinued)
|Amount :||100 µg|
|Isotype :||Rabbit IgG|
|Purification :||Immunoaffinity chromatography|
|Content :||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.|
|Storage condition :||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Immunogen Information :||Peptide CSSGALDYDIPTTASENLYFQ conjugated to KLH|
Well-characterized antibodies against short-sequence epitope tags are common in the study of protein expression in several different expression systems. The TAP (tandem affinity purification)-tag allows rapid purification of complexes from a relatively small number of cells even if the researcher does not have prior knowledge of the complexs composition, activity, or function. Combined with mass spectrometry, the TAP-tag strategy enables the identification of proteins interacting with a given target protein1. Rabbit Anti-TAP-tag Polyclonal Antibody is generated against the common tag, which is composed of a 21-residue peptide, CSSGALDYDIPTTASENLYFQ, derived from the C-terminus of the TAP-tag construct after TEV cleavage.
ELISA: 0.02-0.1 µg/ml
Western blot: 1-2 µg/ml
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.