Anti-Helios Monoclonal Antibody (Clone:22F6)
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Format : | Purified |
Amount : | 0.1 mg |
Isotype : | Hamster IgG |
Purification : | Purified by protein-A affinity chromatography |
Storage condition : | Store at 2-8°C. Do not freeze. |
Flow Cytometry Staining method:
- Perform staining of cell surface markers (CD25, CD4 etc.) for 20 min. at room temperature in the dark; 100 µl of peripheral blood.
- Add 3 ml of PBS with 1% BSA, centrifugate at 300g and discard the supernatant. Further steps perform on ice and with ice-cold reagents.
- Resuspend the cells in 5 ml of cold fixation solution (Miltenyi Biotec) and incubate for 30 min. on ice.
- Centrifugate for 5 min. at 1000 g, 4
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Nucleus |
BioGrid: | 116485. 20 interactions. |
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