Anti-Human CD369 APC MAb(Clone :15E2)
Fig 1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD369 (15E2) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
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| Amount : | 100 tests |
| Isotype : | Mouse IgG2a kappa |
| Purification : | Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Content : | Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azid |
| Storage condition : | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Specificity: The mouse monoclonal antibody 15E2 recognizes an extracellular epitope LWEDGSTFSSN of human CD369 (Dectin-1), a 33 kDa transmembrane glycoprotein expressed predominantly on dendritic cells.
CD369 (dectin-1, beta-glucan receptor) is a 33 kDa type II transmembrane glycoprotein of lectin family, and serves as a part of innate immunity system by binding to beta-glucan polymers, which are typical for yeast and mycobacterial cell walls. CD369 is expressed predominantly on dendritic cells, but it can be detected also on monocytes, macrophages, mast cells, eosinophils, B cells, endothelial cells, and sometimes also on some T cell subsets.
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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