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  7. Anti-Human IgE APC MAb(Clone :4H10)

Anti-Human IgE APC MAb(Clone :4H10)

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Fig 1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human IgE (4H10) APC antibody (concentration in sample 9 μg/ml).

Anti-Human IgE APC MAb(Clone :4H10)
Anti-Human IgE APC MAb(Clone :4H10)
Anti-Human IgE APC MAb(Clone :4H10)

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Fig 1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human IgE (4H10) APC antibody (concentration in sample 9 μg/ml).
Fig 2: Flow cytometry multicolor surface staining of human leukocytes stained using anti-human IgE (4H10) APC antibody (concentration in sample 9 μg/ml) and anti-human CD45 (MEM-28) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
Fig 3: Separation of human IgE positive CD45dim basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human IgE (4H10) APC antibody (concentration in sample 9 μg/ml).

Product code: 30-2864

Clone name : 4H10
Clonality : Monoclonal
Application : FACS
Reactivity : Human
Conjugate : APC

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0.1 mg
$408.00 

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  • Product Info
  • Antigen Details
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Amount : 0.1 mg
Isotype : Mouse IgG1
Purification : Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Content : 0.1 mg/ml
Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage condition : Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen Information : Purified human IgE.

Specificity: The mouse monoclonal antibody 4H10 reacts with human IgE; it recognizes an epitope different from the ones recognized by BE5 and 4G7 antibodies to IgE. The epitope is located within the amino acids 267-279 (TWLEDGQVMDVDL).

Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.

Flow cytometry: Recommended dilution: 0.5-3 µg/ml

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-Human IgE APC MAb(Clone :4H10)

Product code: 30-2864
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