Anti-LAT Monoclonal Antibody (Clone:LAT-01)
|Amount :||0.1 mg|
|Isotype :||Mouse IgG1|
|Purification :||Purified by protein-A affinity chromatography|
|Storage condition :||Store at 2-8°C. Do not freeze.|
LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.
Immunoprecipitation: Tyrosine-phosphorylated form of LAT is not optimally recognized.
Flow cytometry: Intracellular staining. Tyrosine-phosphorylated form of LAT is not optimally recognized.
Western blotting: Recommended dilution: 1-2 μg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|Subcellular location:||Cell membrane|
|Post transnational modification:||Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.|
|Tissue Specificity:||Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).|
|BioGrid:||117971. 41 interactions.|