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    7. CpG ODN (2006), TLR9 ligand (Class B)

    CpG ODN (2006), TLR9 ligand (Class B)

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    Fig-1: Induction of human TLR9 activity by CpG ODN 2006.

    CpG ODN (2006), TLR9 ligand (Class B)
    CpG ODN (2006), TLR9 ligand (Class B)
    CpG ODN (2006), TLR9 ligand (Class B)

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    Fig-1: Induction of human TLR9 activity by CpG ODN 2006.
    Fig-2: Abeomics' CpG ODNs did not show any TLR2 agonist activity. TLR2/NF-kB Leeporter™ HEK293 cells (14-127ACL) were treated with various CpG ODNs at 100 ug/ml as well as a positive TLR2 agonist, Pam3CSK4, at 10 ng/ml for 16 h, and luciferase activity was then analyzed.
    Fig-3: Abeomics' CpG ODNs did not show any TLR4 agonist activity. TLR4/IL-8 Leeporter™ HeLa cells (14-124ACL) were treated with various CpG ODNs at 100 ug/ml as well as a positive TLR4 agonist, LPS, at 10 ng/ml for 16 h, and luciferase activity was then analyzed.

    Product code: 15-1017

    Application : Functional Assay
    Reactivity : Human

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    •  0.1 mg

    • $409.00 

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    Amount : 0.1 mg
    Content : 1 mg/ml in endotoxin-free water
    Storage condition : Upon receipt, store at -20°C (Stable for at least 6 months). Avoid frequent freeze/thaw cycles.
    Sequence:  5'-tCGtCGttttgtCGttttgtCGtt-3' (Class B ODN 2006: Bases are phosphorothioate.)

    Synthetic oligodeoxynucleotides (ODN) containing unmethylated deoxycytosine-deoxyguanosine (CpG) motif are equivalent to bacterial DNA in the immunostimulatory activity, which can induce innate immunity via Toll-like receptor 9 (TLR9) in mammals. There are three major classes of CpG ODNs, and each ODN class exhibits different stimulatory effects on immune cell activation. Class A ODNs are potent inducers of IFN-alpha that leads to the plasmacytoid dendritc cell (pDC) maturation. Class B ODNs are relatively weak inducers of type I IFNs but strong stimulators of human B cells and monocyte maturation. Class C ODNs combine elements of both Classes A and B ODNs, which can induce IFN-alpha in pDC and activation of B cells.

    A. CpG ODN 2006-mediated human TLR9 activation in TLR9/NF-kB Leeporter™ – HEK293 cells (Figure 2). 
     
    1. Harvest TLR9/NF-kB Leeporter™ – HEK293 cells and seed cells into a white solid-bottom 96-well microplate in 100 ul of growth medium at 5 x 104 cells/well.
     
    2. Incubate cells at 37oC in a CO2 incubator for overnight.
     
    3. The next day, stimulate cells with various amounts of CpG ODN 2006. 
     
    4. Incubate at 37oC in a CO2 incubator for 6-16 hours.
     
    5. Add 30-50 ul of luciferase assay reagent per well. 
     
    6. Incubate at room temperature for 1-5 minutes and measure luminescence using a microplate luminometer.
     
     
     

     

    For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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    CpG ODN (2006), TLR9 ligand (Class B)

    Product code: 15-1017
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