G alpha 15 Stable Cell Line-PAC1-CHO-K1-Human
Figure 1: Functional analysis of PAC1 receptor.
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| Amount : | 2 vials |
| Content : | Each Vial contains cells in 45% culture medium, 45% FBS, and 10% DMSO |
| Storage condition : | Immediately upon receipt, store in liquid nitrogen. |
| Alternative Name : | Human Recombinant Vasoactive Intestinal Polypeptide Receptor Stable Cell Line |
The Vasoactive intestinal Peptide/Pituitary Adenylate Cyclase Activating Polypeptide (VPAC) receptor is G protein-coupled and has been divided into at least three types: PAC1, VPAC1, and VPAC2. High expression of PAC1 is observed in CNS and the adrenal medulla. Several splice variants of PAC1 result in proteins that differ at the N-terminus and third intracellular loop; these variants differ in their affinities for PACAP and abilities to activate Gq and Gs. This manual describes establishment of a cell line and a protocol of pharmacologically validated human PAC1 GPCR receptor (Genebank Accession Number: NM_003382).
Application: Screen for antibodies through Flow Cytometry, Immunocytochemistry or Western blotting and analyzing receptors. Culture Medium: Ham's F12, 10% FBS, 100 µg/ml Hygromycin B, 400 µg/ml G418
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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