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    7. GFP/CHO-K1 Stable Cell Line

    GFP/CHO-K1 Stable Cell Line

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    Fig-1: Analysis of the GFP/CHO-K1 stable cell line through fluorescence microscopy. Bright-field image (Left); Fluorescence image (Right).

    GFP/CHO-K1 Stable Cell Line
    GFP/CHO-K1 Stable Cell Line

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    Fig-1:  Analysis of the GFP/CHO-K1 stable cell line through fluorescence microscopy. Bright-field image (Left); Fluorescence image (Right).
    Fig-2: Detection of GFP in the GFP/CHO-K1 stable cell line through flow cytometry . Parental CHO-K1 cells (Green); GFP/CHO-K1 cells (Red).

    Product code: 14-901ACL

    Application : Functional Assay, FACS

    Shipping Info:

    Order now and get it on Tuesday August 16, 2022

    Same day delivery FREE on San Diego area orders placed by 1.00 PM

    Local delivery areas

    Same-day delivery in San Diego available for the following zip codes:

    92121, 92122, 92037, 92117, 92109, 92110, 92101, 92010, 92011, 92009, 92008
    Download TDS / Manual MSDS
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    Size
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    1 vial
    $2,750.00 

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    Shipping Info:

    Order now and get it on Tuesday August 16, 2022

    Same day delivery FREE on San Diego area orders placed by 1.00 PM

    Download TDS / Manual MSDS

    • Product Info
    • Description
    • Application Note
    • Review   (0)
    Amount : 1 vial
    Content : Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO
    Storage condition : Immediately upon receipt, store in liquid nitrogen.

    GFP/CHO-K1 Stable Cell Line is  a stably transfected CHO-K1 cell line which expresses enhanced green fluorescent protein (eGFP).

    Sequence data: Amino acid sequence of eGFP

    MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
    TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE
    RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNY
    NSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGP
    VLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK

     

    Application:.

    •  Screen for GFP through Flow Cytometry. 
    •  Screen for GFP through Fluorescence Microscopy.

    Culture conditions:

    Cells should be grown at 37oC with 5% CO2 using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate) supplemented with 10% heat-inactivated FBS and 1% Pen/Strep, plus 5 µg/ml of Puromycin.
     
    It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a 37oC water-bath, transfer to a tube containing 10 ml of growth medium without Puromycin, spin down cells, resuspend cells in pre-warmed growth medium without Puromycin, transfer resuspended cells to T25 flask and culture in 37oC-CO2 incubator.
     
    Leave the T25 flask in the incubator for 1~2 days without disturbing or changing the medium until cells completely recover viability and become adherent. Once cells are over 90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation. At first passage, switch to growth medium containing Puromycin. Cells should be split before they reach complete confluence.
     
    To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly. To achieve satisfactory results, cells should not be passaged over 16 times.
     
     
     

    LIMITED USE RESTRICTIONS:

    THIS PRODUCT IS SOLELY FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.

    By use of this product, user agrees to be bound by the terms of this limited use statement.

    This product is solely for Internal Research Purposes and not for Commercial Purposes. Commercial Purposes include, but are not limited to (1) use of the cell line in manufacturing; (2) use of the cell line to provide a service, information or data; (3) use of the cell line for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the cell line whether or not such cell lines are resold for use in research. The buyer cannot sell, give or otherwise transfer this product to a third party.

    Commercial License Agreement is available for non-research use if applicable. Please contact Abeomics (info@abeomics.com).

     

     

    For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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    GFP/CHO-K1 Stable Cell Line

    Product code: 14-901ACL
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