Human IL-1β (Interleukin 1 Beta) Pre-Coated ELISA Kit
Fig-1: Human IL-1β Standard Curve
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| Amount : | 1 × 96 Tests |
This assay employs an antibody specific for anti-human IL-1b coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1b present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-1β antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1b bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
| Content : | 1 × 96 well Format (96 tests) |
| Storage condition : | Please refer to the Manual |
This immunoassay kit allows for the in vitro quantitative determination of Human IL-1β concentrations in serum, plasma and other biological fluids.
Detection Range: 7.8 – 500 pg/ml
Sensitivity: < 4.69 pg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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