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    5. IDNK E.Coli, Active

    IDNK E.Coli, Active

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    IDNK E.Coli, Active

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    Product code: 32-6983

    Application : Functional Assay

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    •   2 µg

    •  10 µg

    • $257.00 

    • $325.00 

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    • Product Info
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    Amount : 10 µg
    Purification : Greater than 90.0% as determined by SDS-PAGE.
    Content : The IDNK  solution (1 mg/ml) contains 10% Glycerol, 1mM DTT, 0.15M NaCl and  20mM Tris-HCl buffer (pH 8.0).
    Storage condition : Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
    AA sequence : MGSSHHHHHH SSGLVPRGSH MGSMAGESFI LMGVSGSGKT LIGSKVAALL SAKFIDGDDL HPAKNIDKMS QGIPLSDEDR LPWLERLNDA SYSLYKKNET GFIVCSSLKK QYRDILRKGS PHVHFLWLDG DYETILARMQ RRAGHFMPVA LLKSQFEALE RPQADEQDIV RIDINHDIAN VTEQCRQAVL AIRQNRICAK EGSASDQRCE
    Alternative Name : Thermosensitive gluconokinase, Gluconate kinase 1, idnK, D-gluconate kinase thermosensitive, D-gluconate kinase, thermosensitive, ECK4261, gntV, JW4225, b4268
    Source: Escherichia Coli.
    Sterile Filtered colorless solution.
    D-gluconate kinase or idnk is a thermosensitive protein, consists of 187 a.a and part of the gluconokinase gntK/gntV protein family. Idnk enhances the conversion of ATP + D-gluconate => ADP + 6-phospho-D-gluconate. Idnk has a crucial part in determination of gender, removal of a certain portion of 9p may result in the making of male to female (reversal of sex), that leads to a female that has the genotype of male X, Y.
    IDNK Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 210 amino acids (1-187) and having a molecular mass of 23.4 kDa.IDNK is fused to a 23 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

    Specific activity is >  80unit/mg. One unit will convert 1.0 umole of D-gluconate to 6-phospho-Dgluconate per minute at pH 8.0 at 37°C.

    For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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    IDNK E.Coli, Active

    Product code: 32-6983
    *specify in mg or ml

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