Monoclonal Antibody to GAP43 (Clone: 3H14)
Figure-1: Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to GAP43, 34-1148, dilution 1:1,000, in red, and costained with chicken pAb to MAP2, 34-1064, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane of the neuronal cells, while the MAP2 antibody stains dendrites and perikarya of neurons.
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|Amount :||100 µl|
|Isotype :||Mouse IgM|
|Content :||Purified antibody at 1mg/ml in 50% PBS, 50% glycerol plus 5mM NaN3|
|Storage condition :||Stable at 4°C for one year, for longer term store at -20°C|
GAP43 is an abundant protein which is found heavily concentrated in developing neurons, in particular at the growing tips, the growth cones. One group discovered it since it becomes unregulated during the regeneration of the toad optic nerve, and named it “growth associated protein 43”, the 43 referring to the apparent molecular weight on SDS-PAGE gels (1). Due to unusual mobility on SDS-PAGE GAP43 was independently discovered by several other groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57, the numbers 46, 50 and 57 reflecting the apparent SDS-PAGE molecular weight (2). GAP43 is a major protein kinase C substrate, binds calmodulin avidly and may be anchored to the plasma membrane by reversible palmitoylation on two Cys residues close to the N-terminus (3,4). Knock out of the GAP43 gene in mice is lethal early in postnatal life and is associated with defects in axonal pathfinding (5). GAP43 is one of a large family of “intrinsically disordered proteins” which typically have little defined structure unless they are bound to a more structured partner (6). The 34-1148 antibody was made against the full length recombinant human protein and binds to GAP43 in rodents and other mammalian species. It binds strongly to growth cones and axonal processes of neurons in cell culture and to synaptic regions in sectioned material.
WB: 1:1,000-5,000 IF/ICC and IHC: 1:1,000-5,000
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|Subcellular location:||Cell membrane, Cell projection, Cell junction, Cell projection|
|Post transnational modification:||Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2.|
|BioGrid:||108867. 27 interactions.|