Monoclonal Antibody to mouse MHC Class II (I-A/I-E) (Clone: M5/114)-NALE
Figure-1: Cell surface flow analysis of mouse MHC Class II on C57 mice splenocytes using 0.5 µg/10^6 cells of MHC class II antibody (Clone: M5/114). Green represents isotype control; red represents anti-mouse MHC class II antibody. mouse anti-rat Fitc conjugate was used as secondary antibody.
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| Format : | Low Endotoxin,Azide Free |
| Amount : | 100 µg |
| Isotype : | Rat IgG2b, k |
| Immunogen Information : | Activated C57BL/6 mouse spleen cells was used as Immunogen |
MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
| Purification : | Protein G |
| Content : | PBS, pH 7.0 Contains no stabilizers or preservatives |
| Storage condition : | The antibody solution should be stored undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze. |
- <2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay
- Functional assays, Immunofluorescence, Western blot, Immunoprecipitation, Flow cytometry
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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