Monoclonal antibody to Topo II alpha (Clone: ABM48B4 )
Fig:1- Intracellular Flow analysis of Topo II alpha antibody in Jurkat cells using 0.5 µg/ 10^6 cells of anti-Topo II alpha antibody (ABM48B4). Green represents isotype control;red represents anti-Topo II alpha antibody. Goat anti-mouse PE conjugate was used as secondary antibody.
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|Amount :||100 µg|
|Isotype :||Mouse IgG2b Kappa|
|Purification :||Protein G Chromatography|
|Content :||25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.|
|Storage condition :||Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.|
Topoisomerases are essential nuclear enzymes that are involved in DNA supercoiling regulation and play key roles in transcription, replication, and chromosome segregation. Two major classes of topoisomerases (types I and II) are distinguished by the number of DNA strands that they cleave and the mechanism by which they alter the topological properties of DNA. Topo II Alpha is cell cycle-regulated and is essential for the survival of proliferating cells. It accumulates on chromatin during M-phase, a dynamic localization that is dependent on its C-terminal domain. Although Topo II alpha is the major form of Topo II responsible for decatenation, mitotic chromosome formation and chromosome segregation in proliferating cells, the contribution of the two isoforms has not yet been fully established. Topo II Alpha plays an important role in DNA synthesis and transcription and has been implicated in a variety of human cancers. It also plays a role in various cancer subtypes and acts as targets for cancer therapy, as well as biomarkers for prediction of response.
Western blot analysis: 1-2 µg/ml;FACS Analysis: 0.5-1 µg/10^6 cells
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|Subcellular location:||Cytoplasm, Nucleus|
|Post transnational modification:||Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.|
|BioGrid:||113006. 107 interactions.|