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    5. PGAM1 Human, Active

    PGAM1 Human, Active

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    PGAM1 Human, Active

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    Product code: 32-6878

    Application : Functional Assay

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    •   2 µg

    •  10 µg

    • $257.00 

    • $325.00 

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    • Product Info
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    Amount : 10 µg
    Purification : Greater than 90% as determined by SDS-PAGE.
    Content : The PGAM1 1mg/ml protein solution contains 20mM Tris-HCl pH-8, 1mM DTT, and 10% glycerol.
    Storage condition : Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
    AA sequence : MGSSHHHHHH SSGLVPRGSH MAAYKLVLIR HGESAWNLEN RFSGWYDADL SPAGHEEAKR GGQALRDAGY EFDICFTSVQ KRAIRTLWTV LDAIDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEA QVKIWRRSYD VPPPPMEPDH PFYSNISKDR RYADLTEDQL PSCESLKDTI ARALPFWNEE IVPQIKEGKR VLIAAHGNSL RGIVKHLEGL SEEAIMELNL PTGIPIVYEL DKNLKPIKPM QFLGDEETVR KAMEAVAAQG KAKK.
    Alternative Name : Phosphoglycerate mutase isozyme B, PGAM-B, PGAMA.
    Source: Escherichia Coli.
    Sterile Filtered clear colorless solution.
    PGAM1 is part of the phosphoglycerate mutase family. PGAM1 is an essential component of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. PGAM1 is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). PGAM1 mutations lead to muscle phosphoglycerate mutase deficiency, a.k.a. glycogen storage disease X.
    PGAM1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 274 amino acids (1-254 a.a.) and having a molecular mass of 30.9 kDa. The PGAM1 is fused to a 20 amino acid His Tag at N-Terminus and purified by proprietary chromatographic techniques.

    Specific activity is >300 units/mg, in which One unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.

    For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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    PGAM1 Human, Active

    Product code: 32-6878
    *specify in mg or ml

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