Polyclonal Antibody to Neurofilament NF-H

Name: Polyclonal Antibody to Neurofilament NF-H
Brand: Abeomics
SKU: 34-1078
Price: 462.0000 USD
Availability: InStock
Rating: 5 (10 reviews)

Figure-1: Immunohistological analysis of a mouse hippocampus section stained with rabbit pAb to NF-H,(34-1078), dilution 1:2,000 in red, and costained with mouse mAb to myelin basic protein (MBP),(34-1072), dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45Î¼M, and free-floating sections were stained with above antibodies. The NF-H antibody labels a network of axons of different neurons, while the MBP antibody stains myelin sheath around these axons.

Polyclonal Antibody to Neurofilament NF-H

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Figure-1: Immunohistological analysis of a mouse hippocampus section stained with rabbit pAb to NF-H,(34-1078), dilution 1:2,000 in red, and costained with mouse mAb to myelin basic protein (MBP),(34-1072), dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45Î¼M, and free-floating sections were stained with above antibodies. The NF-H antibody labels a network of axons of different neurons, while the MBP antibody stains myelin sheath around these axons.

Figure-2: Western blot analysis of different tissue lysates using rabbit pAb to NF-H,(34-1078), dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord [4] mouse brain, and [5] mouse spinal cord lysate. Strong band at about 220kDa corresponds to the phosphorylated axonal form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected with(34-1078) antibody.

Product code: 34-1078

Clonality :	Polyclonal
Application :	WB
Reactivity :	Human

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Sera
Amount :	100 µl
Isotype :	Rabbit, IgG
Content :	Antibody is supplied as an aliquot of serum
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	NEFH
Gene ID :	4744
Uniprot ID :	P12036
Alternative Name :	200 kDa neurofilament protein, Neurofilament triplet H protein
Immunogen Information :	Native NF-H purified from bovine spinal cord

WB: 1:10,000-25,000. ICC/IF and IHC: 1:1,000-5,000.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm
Post transnational modification:	Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
BioGrid:	110819. 25 interactions.