Protein A Monoclonal Antibody (Clone: ABM4B3.1G3)
Figure-1: Western blot analysis of Protein A. Anti-Protein A antibody (Clone: ABM4B3.1G3) was used at 0.1 µg/ml on Recombinant protein.
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Mouse IgG2b, kappa |
| Purification : | Protein G Chromatography |
| Content : | 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
| Gene : | spa |
| Immunogen Information : | Protein A from Staphylococcus aureus |
Protein A (PA) is a 42 kDa surface protein originally found in the cell wall of the Gram positive bacteria Staphylococcus aureus. It has the specific ability to bind with the constant (Fc) portion of immunoglobulin molecules from various different species. Protein A is encoded by the polymorphic X-region and functions as an immunoglobulin-binding protein, characteristically it has the ability to bind with the ‘Fc-region’ of IgG.
Due to its high affinity for Fc region of human IgG, it is extensively used in affinity chromatography process required for the purification of antibodies. However, one of the major disadvantages of this method of purification is co elution or leaching of PA along with the target antibody.
Recommended dilutions: WB: 0.1-1 µg/ml, ELISA: 1 µg/ml. However, this need to be optimized based on the research applications.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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