Rabbit Polyclonal Antibody to ERK2(Discontinued)
|Amount :||40 µg|
|Isotype :||Rabbit IgG|
|Purification :||Immunoaffinity chromatography|
|Content :||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide|
|Storage condition :||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Gene ID :||5594|
|Uniprot ID :||P28482|
|Alternative Name :||ERK2 pAb, ERT1, Extracellular signal-regulated kinase 2, MAP kinase isoform p42, Mitogen-activated protein kinase 2, ERK2, PRKM1, PRKM2|
|Immunogen Information :||KLH-coupled synthetic peptide corresponding to 300-322 residues of human ERK2 .|
ERK (extracellular signal regulated kinase), also known as MAPK (mitogen activated protein kinase) includes two closely related isoforms ERK1 and ERK2 (p44 and p42 MAP Kinase). ERK1/2 signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines. The downstream effects of ERK1/2 signaling pathway are linked to the regulation of cell growth and differentiation as well as the cytoskeleton. Downstream targets of ERK1/2 contain p90RSK and the transcription factor Elk-1. ERK1 and ERK2 are phosphorylated within the activation loop on both a threonine and a tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1/2. ERK1/2 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases. Rabbit Anti-ERK2 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide (KRIEVEQALAHPYLEQYYDPSDE) corresponding to 300-322 residues of human ERK2 (Swiss Prot: P28482).
ELISA: 0.05-0.2 µg/ml
Western blot: 1-2 µg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.