Recombinant Anti-Monkeypox A33 Antibody (Clone: MPXV-56)
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| Amount : | 100 µg (1mg/mL) |
| Isotype : | Human IgG1 |
| Purification : | ≥95% monomer by analytical SEC |
| Content : | 1.0 mg/ml. This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Storage condition : | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -70°C. Avoid Repeated Freeze Thaw Cycles. |
| Alternative Name : | MPXV |
| Immunogen Information : | MPXV-56 was generated from peripheral blood mononuclear cells obtained from donors who had either recovered from naturally-occurring MPXV infection or been immunized against vaccinia. |
Expression Host : HEK-293
Endotoxin Level : ≤ 1.0 EU/mg as determined by the LAL method
Specificity : Clone MPXV-56 is reactive against A33, a surface antigen on the EV form of the virus, and is of the IgG2 isotype.
Complementary screening approaches were used to identify orthopoxvirus-specific mAbs to MPXV, cowpox virus (CPXV), variola virus (VARV), and vaccinia virus (VACV). MPXV-56 is reactive against VACV antigen and lysate, CPXV lysate, MPXV lysate, and VARV antigen and lysate. MPXV-56 binds to VACV and VARV purified antigen and weakly binds virus-infected cell lysates of VACV, CPXV, and MPXV. MPXV-56 neutralizes VACV EV plus complement (+C’) but not MV, MV+C’, or EV.
MPXV-56 also neutralizes CPXV EV+C’ but not EV and weakly neutralizes MPXV EV+C’ but not MV, MV+C’, or EV.
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