Recombinant Rabbit Monoclonal Antibody to MART-1 / Melan-A / MLANA (Melanoma Marker)(Clone : MLANA/1761R)
|Purification :||Purified Ab with BSA and Azide at 200ug/ml|
|Content :||200ug/ml of Ab purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.|
|Storage condition :||Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.|
MW : 20-22kDa (doublet); Positive Control : SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas;Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Western Blotting (0.5-1.0ug/ml); Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|Subcellular location:||Endoplasmic reticulum membrane, Golgi apparatus, Golgi apparatus, Melanosome|
|Post transnational modification:||Acylated.|
|Tissue Specificity:||Expression is restricted to melanoma and melanocyte cell lines and retina.|
|BioGrid:||108604. 2 interactions.|