Recombinant SARS-CoV-2 Spike RBD Protein, mFc tag
Fig 1 : Recombinant 2019-nCoV Spike RBD Protein with mFc tag was determined by SDS-PAGE with Coomassie Blue, showing a band at 60 kDa.
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| Amount : | 100 µg |
| Purification : | >95% by SDS-PAGE. |
| Content : | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4. |
| Storage condition : | Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. This product is stable at ≤ -70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. |
| AA sequence : | The target protein is expressed with sequence (Arg319-Phe541) of 2019-ncov Spike RBD (Accession #YP_009724390.1) fused with a mFc tag at the C-terminus. |
| Gene ID : | 43740568 |
| Alternative Name : | S1-RBD protein; NCP-CoV RBD Protein; novel coronavirus RBD Protein; 2019-nCoV RBD Protein; S glycoprotein Subunit1 RBD Protein |
Source: HEK293 cells.
Recombinant 2019-nCoV Spike RBD Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of 2019-ncov Spike RBD (Accession #YP_009724390.1) fused with a mFc tag at the C-terminus.
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 Protein at 2μg/mL (100 μL/well) can bind Recombinant 2019-nCoV Spike RBD-mFc Protein，the EC50 of Recombinant 2019-nCoV Spike RBD-mFc Protein is 2.58 ng/mL.
Endotoxin: < 0.1 EU/μg of the protein by LAL method.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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