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  5. Recombinant SARS-CoV-2 Spike RBD Protein, mFc tag

Recombinant SARS-CoV-2 Spike RBD Protein, mFc tag

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Fig 1 : Recombinant 2019-nCoV Spike RBD Protein with mFc tag was determined by SDS-PAGE with Coomassie Blue, showing a band at 60 kDa.

Recombinant SARS-CoV-2 Spike RBD Protein, mFc tag
Recombinant SARS-CoV-2 Spike RBD Protein, mFc tag
Recombinant SARS-CoV-2 Spike RBD Protein, mFc tag

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Fig 1 : Recombinant 2019-nCoV Spike RBD Protein with mFc tag was determined by SDS-PAGE with Coomassie Blue, showing a band at 60 kDa.
Fig 2 :Immobilized Human ACE2 on COOH Chip, can bind 2019-nCoV Spike S1 with an affinity constant of 6.58 nM as determined in a SPR assay (Nicoya OpenSPR).
Fig 3 :Immobilized Recombinant Human ACE2 Protein at 2μg/mL (100 μL/well) can bind Recombinant 2019-nCoV Spike RBD-mFc Protein,the EC50 of Recombinant 2019-nCoV Spike RBD-mFc Protein is 2.58 ng/mL.

Product code: 32-190028

Application : Functional Assay

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$545.00 

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Amount : 100 µg
Purification : >95% by SDS-PAGE.
Content : Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.
Storage condition : Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. This product is stable at ≤ -70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.
AA sequence : The target protein is expressed with sequence (Arg319-Phe541) of 2019-ncov Spike RBD (Accession #YP_009724390.1) fused with a mFc tag at the C-terminus.
Gene ID : 43740568
Alternative Name : S1-RBD protein; NCP-CoV RBD Protein; novel coronavirus RBD Protein; 2019-nCoV RBD Protein; S glycoprotein Subunit1 RBD Protein

Source: HEK293 cells.
Recombinant 2019-nCoV Spike RBD Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of 2019-ncov Spike RBD (Accession #YP_009724390.1) fused with a mFc tag at the C-terminus.

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 Protein at 2μg/mL (100 μL/well) can bind Recombinant 2019-nCoV Spike RBD-mFc Protein,the EC50 of Recombinant 2019-nCoV Spike RBD-mFc Protein is 2.58 ng/mL.

Endotoxin: < 0.1 EU/μg of the protein by LAL method.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Recombinant SARS-CoV-2 Spike RBD Protein, mFc tag

Product code: 32-190028
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