Rabbit Polyclonal Antibody to Caspase 3(Discontinued)
|Amount :||0.5 ml|
|Isotype :||Rabbit IgG|
|Content :||Purified immunoglobulin fraction of rabbit antiserum against Caspase-3 containing sodium azide as preservative.|
|Storage condition :||Store at 2-8°C.|
This antibody reacts with the active form of caspase 3 (17 kD protein). Caspase 3 is a member of the interleukin-1 beta-converting enzyme family and is thought to be associated with the induction of apoptosis. Caspase 3 is synthesized as an inactive 32 kD proenzyme and is processed during apoptosis generating two subunits of 17 kD and 12 kD. Caspase 3 stains the epithelial cells of skin, renal proximal tubules, and collecting ducts.
IHC : 1:50-1:100
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|Post transnational modification:||S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.|
|Tissue Specificity:||Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.|
|BioGrid:||107286. 102 interactions.|