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  5. Recombinant SARS-CoV-2 Spike S1 Protein (His Tag)

Recombinant SARS-CoV-2 Spike S1 Protein (His Tag)

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Figure-1: Recombinant COVID-19 Spike S1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 110-130 kDa.

Recombinant SARS-CoV-2 Spike S1 Protein (His Tag)
Recombinant SARS-CoV-2 Spike S1 Protein (His Tag)

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Figure-1: Recombinant COVID-19 Spike S1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 110-130 kDa.
Figure-2: Measured by its binding ability in a functional ELISA.Immobilized Recombinant Human ACE2 at 
 2 μg/mL (100μl/well ) can bind Recombinant nCoV-S1, The EC50 of nCoV-S1 is 0.25-0.45 μg/mL.

Product code: 32-190020

Application : Functional Assay

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$483.00 

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Amount : 100 μg
Purification : >90% by SDS-PAGE.
Content : Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.
Storage condition : Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. Avoid repeated freeze/thaw cycles.
AA sequence : The target protein is expressed with sequence (Val16-Arg685) of 2019-ncov Spike S1fused with a 6×His tag at the C-terminus.
Gene ID : 43740568
Alternative Name : S1 protein; Spike glycoprotein Subunit1;S glycoprotein Subunit1;Spike protein S1;novel coronavirus s1 Protein

Source: HEK 293 cells. The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.

Bio-Activity: Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system. Measured by its binding ability in a functional ELISA.Immobilized Recombinant Human ACE2 at 2 g/mL (100L/well ) can bind Recombinant nCoV-S1, The EC50 of nCoV-S1 is 0.25-0.45 μg/mL. Endotoxin : < 1.0 EU/μg of the protein by LAL method.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Recombinant SARS-CoV-2 Spike S1 Protein (His Tag)

Product code: 32-190020
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