Recombinant SARS-CoV-2 Spike S1 Protein (His Tag)
Figure-1: Recombinant COVID-19 Spike S1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 110-130 kDa.
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| Amount : | 100 μg |
| Purification : | >90% by SDS-PAGE. |
| Content : | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. |
| Storage condition : | Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. Avoid repeated freeze/thaw cycles. |
| AA sequence : | The target protein is expressed with sequence (Val16-Arg685) of 2019-ncov Spike S1fused with a 6×His tag at the C-terminus. |
| Gene ID : | 43740568 |
| Alternative Name : | S1 protein; Spike glycoprotein Subunit1;S glycoprotein Subunit1;Spike protein S1;novel coronavirus s1 Protein |
Source: HEK 293 cells. The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.
Bio-Activity: Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system. Measured by its binding ability in a functional ELISA.Immobilized Recombinant Human ACE2 at 2 g/mL (100L/well ) can bind Recombinant nCoV-S1, The EC50 of nCoV-S1 is 0.25-0.45 μg/mL. Endotoxin : < 1.0 EU/μg of the protein by LAL method.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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