Monoclonal Antibody to SARS-CoV-2 Nucleocapsid (Clone: ABM1F11.1E1)
Figure-1: An indirect ELISA is carried out by coating nucleocapsid protein in serial dilution from 20 ng to 0.312 ng and using 100 ng of purified monoclonal antibodies 1F11.1E1. Peroxidase conjugated Goat-Anti mouse antibody was used at 1:5000 dilution.
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The structural nucleocapsid (N) protein of nCoV/SARS-CoV-2/COVID-19 is a predicted 46 kDa phosphoprotein having 419 amino acid residues. A short Serine rich stretch and a recognized nuclear localization signal are the unique features of the nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19, which seems to have a little homology with the proteins of other members of this large corona virus family. These features also suggest the involvement of nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19 in different stages of viral lifecycle. The protein has multifaceted activities including packing of the nCoV/SARS-CoV-2/COVID-19 viral genome into a helical ribonucleocapsid (RNP) and playing an important role in viral self-assembly causing nCoV/SARS-CoV-2/COVID-19. The nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19 also forms dimer in the cell by self-association with the help of interactive C terminal domain.
| Purification : | Protein G Chromatography |
| Content : | 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
Recommended dilutions: WB: 0.1-1 µg/ml, ELISA: 1 µg/ml. However, this need to be optimized based on the research applications.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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