Monoclonal Antibody to SARS-CoV-2 Nucleocapsid (Clone: ABM1F11.1E1)

Figure-1: An indirect ELISA is carried out by coating nucleocapsid protein in serial dilution from 20 ng to 0.312 ng and using 100 ng of purified monoclonal antibodies 1F11.1E1. Peroxidase conjugated Goat-Anti mouse antibody was used at 1:5000 dilution.

Monoclonal Antibody to SARS-CoV-2 Nucleocapsid (Clone: ABM1F11.1E1)

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Figure-1: An indirect ELISA is carried out by coating nucleocapsid protein in serial dilution from 20 ng to 0.312 ng and using 100 ng of purified monoclonal antibodies 1F11.1E1. Peroxidase conjugated Goat-Anti mouse antibody was used at 1:5000 dilution.

Figure-2: Western Blot analysis: The purified antibodies 1F11.1E1 was tested on Nucleocapsid Recombinant protein at different concentrations, 0.1 (lane 1), 0.5 (lane 2), and 1.0 Î¼g/ml (lane 3), (4) RBD protein, (5)unrelated protein 1, (6) unrelated protein 2, (7) unrelated protein 3, to detect the specific binding. 25 ng of proteins was loaded per lane.

Figure-3: Western Blot analysis of SARS-CoV-2 Nucleocapsid Antibody: Anti- SARS-CoV-2 Nucleocapsid Antibody (Clone: ABM1F11.1E1) was used at 2 µg/ml on (1) SARS-CoV-2 virus infected Vero Cell lysate and (2) Mock infected lysate.

Product code: 10-10032

Clone name :	ABM1F11.1E1
Clonality :	Monoclonal
Application :	ELISA, WB
Reactivity :	Human

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Format :	Purified
Amount :	100 µg
Isotype :	Mouse IgG1, Kappa
Purification :	Protein G Chromatography
Content :	25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition :	Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	N
Gene ID :	43740575
Uniprot ID :	P0DTC9
Immunogen Information :	Full length recombinant SARS-CoV-2 nucleocapsid Protein was used as the immunogen for this antibody.

The structural nucleocapsid (N) protein of nCoV/SARS-CoV-2/COVID-19 is a predicted 46 kDa phosphoprotein having 419 amino acid residues. A short Serine rich stretch and a recognized nuclear localization signal are the unique features of the nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19, which seems to have a little homology with the proteins of other members of this large corona virus family. These features also suggest the involvement of nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19 in different stages of viral lifecycle. The protein has multifaceted activities including packing of the nCoV/SARS-CoV-2/COVID-19 viral genome into a helical ribonucleocapsid (RNP) and playing an important role in viral self-assembly causing nCoV/SARS-CoV-2/COVID-19. The nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19 also forms dimer in the cell by self-association with the help of interactive C terminal domain.

Recommended dilutions: WB: 0.1-1 µg/ml, ELISA: 1 µg/ml. However, this need to be optimized based on the research applications.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Leekha A, Saeedi A, Sefat KMSR, Kumar M, Martinez-Paniagua M, Damian A, Kulkarni R, Reichel K, Rezvan A, Masoumi S, Liu X, Cooper LJN, Sebastian M, Sands CM, Das VE, Patel NB, Hurst B, Varadarajan N.
Multi-antigen intranasal vaccine protects against challenge with sarbecoviruses and prevents transmission in hamsters.
Nat Commun. 2024 Jul 23;15(1):6193. doi: 10.1038/s41467-024-50133-2.
PMID: 39043645; PMCID: PMC11266618.