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  7. Monoclonal Antibody to SARS-CoV-2 Nucleocapsid (Clone: ABM1F11.1E1)

Monoclonal Antibody to SARS-CoV-2 Nucleocapsid (Clone: ABM1F11.1E1)

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Figure-1: An indirect ELISA is carried out by coating nucleocapsid protein in serial dilution from 20 ng to 0.312 ng and using 100 ng of purified monoclonal antibodies 1F11.1E1. Peroxidase conjugated Goat-Anti mouse antibody was used at 1:5000 dilution.

Monoclonal Antibody to SARS-CoV-2 Nucleocapsid  (Clone: ABM1F11.1E1)
Monoclonal Antibody to SARS-CoV-2 Nucleocapsid  (Clone: ABM1F11.1E1)
Monoclonal Antibody to SARS-CoV-2 Nucleocapsid  (Clone: ABM1F11.1E1)

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Figure-1: An indirect ELISA is carried out by coating nucleocapsid protein in serial dilution from 20 ng to 0.312 ng and using 100 ng of purified monoclonal antibodies 1F11.1E1. Peroxidase conjugated Goat-Anti mouse antibody was used at 1:5000 dilution.
Figure-2: Western Blot analysis: The purified antibodies 1F11.1E1 was tested on Nucleocapsid Recombinant protein at different concentrations, 0.1 (lane 1), 0.5 (lane 2), and 1.0 μg/ml (lane 3), (4) RBD protein, (5)unrelated protein 1, (6) unrelated protein 2,  (7) unrelated protein 3, to detect the specific binding. 25 ng of proteins was loaded per lane.
Figure-3: Western Blot analysis of SARS-CoV-2 Nucleocapsid Antibody: Anti- SARS-CoV-2 Nucleocapsid Antibody (Clone: ABM1F11.1E1) was used at 2 µg/ml on (1) SARS-CoV-2 virus infected Vero Cell lysate and (2) Mock infected lysate.

Product code: 10-10032

Clone name : ABM1F11.1E1
Clonality : Monoclonal
Application : ELISA, WB
Reactivity : Human

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Order now and get it on Tuesday June 06, 2023

Same day delivery FREE on San Diego area orders placed by 1.00 PM

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Same-day delivery in San Diego available for the following zip codes:

92121, 92122, 92037, 92117, 92109, 92110, 92101, 92010, 92011, 92009, 92008
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Available Pack Size(s)

  •   25 µg

  •  100 µg

  • $150.00 

  • $380.00 

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Shipping Info:

Order now and get it on Tuesday June 06, 2023

Same day delivery FREE on San Diego area orders placed by 1.00 PM

Download TDS MSDS

  • Product Info
  • Antigen Details
  • Description
  • Application Note
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Format : Purified
Amount : 100 µg
Isotype : Mouse IgG1, Kappa
Purification : Protein G Chromatography
Content : 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Gene : N
Gene ID : 43740575
Uniprot ID : P0DTC9
Immunogen Information : Full length recombinant SARS-CoV-2 nucleocapsid Protein was used as the immunogen for this antibody.

The structural nucleocapsid (N) protein of nCoV/SARS-CoV-2/COVID-19 is a predicted 46 kDa phosphoprotein having 419 amino acid residues. A short Serine rich stretch and a recognized nuclear localization signal are the unique features of the nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19, which seems to have a little homology with the proteins of other members of this large corona virus family. These features also suggest the involvement of nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19 in different stages of viral lifecycle. The protein has multifaceted activities including packing of the nCoV/SARS-CoV-2/COVID-19 viral genome into a helical ribonucleocapsid (RNP) and playing an important role in viral self-assembly causing nCoV/SARS-CoV-2/COVID-19. The nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19 also forms dimer in the cell by self-association with the help of interactive C terminal domain.

Recommended dilutions: WB: 0.1-1 µg/ml, ELISA: 1 µg/ml. However, this need to be optimized based on the research applications.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Monoclonal Antibody to SARS-CoV-2 Nucleocapsid (Clone: ABM1F11.1E1)

Product code: 10-10032
*specify in mg or ml

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