STAT3 Leeporter™ GFP Reporter-HEK293 Cell Line
Fig-1: Induction of STAT3 activity by IL-6 in STAT3 Leeporter™ GFP reporter-HEK293 cells was analyzed by fluorescence microscopy. IL-6 was treated at 0.01, 0.1, 1, 10 and 100 ng/ml as noted.
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Shipping Info:
Order now and get it on Thursday December 12, 2024
Same day delivery FREE on San Diego area orders placed by 1.00 PM
Amount : | 1 Vial |
Content : | Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO. |
Storage condition : | Immediately upon receipt, store in liquid nitrogen. |
The STAT3 Leeporter™ GFP Reporter cell line is a stably transfected HEK293 cell line, which expresses enhanced green fluorescent protein (eGFP) reporter gene under the transcriptional control of the STAT3 responsive promoter, so that the cell line is designed to measure the transcriptional activity of STAT3. As a transcription factor, Signal Transducer and Activator of Transcription 3 (STAT3) is activated through phosphorylation at tyrosine 705 in response to various cytokines including IL-6, interferons, epidermal growth factor, hepatocyte growth factor and leukemia inhibitory factor. The phosphorylated STAT3 forms homodimers or heterodimers with STAT1, and the dimers translocate to nucleus in which DNA binding/promoter induction occurs. The cell line is designed to analyze by fluorescence microscopy and flow cytometry. The STAT3 induction by IL-6 is shown in Figures 1 and 2.
Application:
- Monitor the STAT3 signaling pathway activity.
- Screen for activators or inhibitors of the STAT3 signaling pathway.
Culture conditions:
To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly. To achieve satisfactory results, cells should not be passaged over 16 times.
Functional validation:
A. Response of STAT3 Leeporter™ GFP reporter – HEK293 cells to IL-6.
LIMITED USE RESTRICTIONS:
THIS PRODUCT IS SOLELY FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.
By use of this product, user agrees to be bound by the terms of this limited use statement.
This product is solely for Internal Research Purposes and not for Commercial Purposes. Commercial Purposes include, but are not limited to (1) use of the cell line in manufacturing; (2) use of the cell line to provide a service, information or data; (3) use of the cell line for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the cell line whether or not such cell lines are resold for use in research. The buyer cannot sell, give or otherwise transfer this product to a third party.
Commercial License Agreement is available for non-research use if applicable. Please contact Abeomics ([email protected]).
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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