Anti-5-bromodeoxyuridine (BrdU) Monoclonal Antibody (Clone:Bu20a)-APC Conjugated
Figure 1: Flow cytometry intracellular staining pattern of K562 cells cultivated with BrdU stained using anti-BrdU (Bu20a) APC antibody (concentration in sample 8 μg/ml).
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| Amount : | 100 tests (T100) |
| Isotype : | Mouse IgG1 |
| Purification : | Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Content : | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Storage condition : | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Gene ID : | 35 |
| Alternative Name : | 5-bromodeoxyuridine |
| Immunogen Information : | Bromodeoxyuridine conjugated to BSA |
Specificity: The antibody Bu20a reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunohistochemistry staining. It cross-reacts with EdU.
Description: Bromodeoxyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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