Anti-Biotin Monoclonal Antibody(1D4-C5)-HRP Conjugated
Figure 1: ELISA analysis of biotin by mouse monoclonal antibody 1D4-C5 and Streptavidin. MaxiSorp plate was coated with biotin-labeled monoclonal antibody, then blocked with BSA, and incubated with dilution series of anti-biotin HRP conjugated primary antibody and HRP conjugated Streptavidin for comparison. Colorimetric detection was performed using TMB substrate and absorbance was measured at 450 nm.
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| Amount : | 0.1 mg |
| Isotype : | Mouse IgG2a kappa |
| Purification : | Purified antibody is conjugated with activated horseradish peroxidase (HRP) of high specific enzyme activity under optimum conditions and unconjugated antibody and free HRP are removed by size-exclusion chromatography. |
| Content : | Concentration: 0.2 mg/ml Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 0.01% thimerosal |
| Storage condition : | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Alternative Name : | vitamin H, vitamin B7 |
| Immunogen Information : | biotinylated limulus hemocyanin |
Specificity: Mouse monoclonal antibody 1D4-C5 recognizes biotin and can be used as a second-step reagent for detecting biotinylated antigens.
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