Anti-CD22 / BL-CAM (B-Cell Marker) Monoclonal Antibody(Clone: BLCAM/1795)
Product code: 36-3549
|Clone name :||BLCAM/1795|
|Application :||ELISA, FACS, WB, IF, IHC|
|Gene ID :||933|
|Uniprot ID :||P20273|
|Alternative Name :||B-lymphocyte cell adhesion molecule (BL-CAM); B-cell receptor CD22; CD22; Lectin 2; Lyb8; Sialic acid-binding Ig-like lectin 2 (Siglec-2); SIGLEC2; T-cell surface antigen Leu-14|
|Amount :||100 µg|
|Isotype :||Mouse IgG1, kappa|
|Immunogen Information :||Recombinant fragment (around aa52-178) of human BL-CAM/CD22 protein (exact sequence is proprietary)|
|Content :||200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.|
|Storage condition :||Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.|
ELISA (Use Ab at 2-4µg/ml for coating) (Order Ab without BSA);Flow Cytometry (1-2µg/million cells);Western Blot (1-2µg/ml);Immunofluorescence (1-2µg/ml);Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.