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  7. Anti-CD235ab / Glycophorin A Monoclonal Antibody (Clone:HIR2)

Anti-CD235ab / Glycophorin A Monoclonal Antibody (Clone:HIR2)

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Anti-CD235ab / Glycophorin A Monoclonal Antibody (Clone:HIR2)

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Product code: 30-1288

Clone name : HIR2
Clonality : Monoclonal
Application : FACS
Reactivity : Human

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0.1 mg
$348.00  $278.40 

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For estimated delivery dates, please contact us at support@abeomics.com

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Format : Purified
Amount : 0.1 mg
Isotype : Mouse IgG2b
Purification : Purified by protein-A affinity chromatography
Storage condition : Store at 2-8°C. Do not freeze.
Gene : GYPA
Gene ID : 2993
Uniprot ID : P02724
Alternative Name : GYPA, GPA
Immunogen Information : Synthetic peptide (Human, N-terminal)

CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin.

Flow Cytometry This HIR2 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes and cell agglutination assay and can be used at approximately 0.1 µg per million cells. Immunohistochemistry (frozen sections) Agglutination The antibody HIR2 agglutinates untreated RBCs but failes to agglutinate papain-treated cells.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Cell membrane
Post transnational modification: The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific).
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Anti-CD235ab / Glycophorin A Monoclonal Antibody (Clone:HIR2)

Product code: 30-1288
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