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  7. Anti-CD31 / PECAM-1 Monoclonal Antibody (Clone:MEM-05)

Anti-CD31 / PECAM-1 Monoclonal Antibody (Clone:MEM-05)

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Anti-CD31 / PECAM-1 Monoclonal Antibody (Clone:MEM-05)

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Product code: 30-1171

Clone name : MEM-05
Clonality : Monoclonal
Application : ICC, IHC, FACS, WB
Reactivity : Human

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0.1 mg
$339.00 

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Format : Purified
Amount : 0.1 mg
Isotype : Mouse IgG1
Purification : Purified by protein-A affinity chromatography
Storage condition : Store at 2-8°C. Do not freeze.
Gene : PECAM1
Gene ID : 5175
Uniprot ID : P16284
Alternative Name : PECAM1
Immunogen Information : Leukocytes of patient suffering from LGL-type leukaemia

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

Flow Cytometry Recommended dilution:1-10 µg/ml
Western Blotting Application note: Non-reducing conditions

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Cell junction
Post transnational modification: Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts.
Tissue Specificity: Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells (PubMed:18388311, PubMed:21464369). Expressed in human umbilical vein endothelial cells (HUVECs) (at protein level) (PubMed:19342684, PubMed:17580308). Expressed on neutrophils (at protein level) (PubMed:17580308). Isoform Long predominates in all tissues examined (PubMed:12433657). Isoform Delta12 is detected only in trachea (PubMed:12433657). Isoform Delta14-15 is only detected in lung (PubMed:12433657). Isoform Delta14 is detected in all tissues examined with the strongest expression in heart (PubMed:12433657). Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level) (PubMed:12433657, PubMed:18388311).
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Anti-CD31 / PECAM-1 Monoclonal Antibody (Clone:MEM-05)

Product code: 30-1171
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