Anti-Cytokeratin 18 Monoclonal Antibody (Clone:DC-10)
|Amount :||0.1 mg|
|Isotype :||Mouse IgG1|
|Purification :||Purified by protein-A affinity chromatography|
|Storage condition :||Store at 2-8°C. Do not freeze.|
Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.Cytokeratin 18 belongs to type I family (acidic cytokeratins).
Western blotting: Recommended dilution: 1-2 μg/ml.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: colon, skin.
Immunocytochemistry: positive control: MCF-7 human breast adenocarcinoma cell line, HeLa human cervix carcinoma cell line.
ELISA: Positive control: MCF-7 human breast adenocarcinoma cell line; the antibody DC-10 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04.
Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|Subcellular location:||Cytoplasm, Nucleus|
|Post transnational modification:||O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.|
|Tissue Specificity:||Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.|
|BioGrid:||110073. 113 interactions.|