Fig. 1: Flow Cytometric Analysis of Jurkat cells. CD3e Mouse Monoclonal Antibody (B-B12) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Product code: 36-3476
| Clone name : | B-B12 |
| Application : | FACS, IF |
| Reactivity : | Human |
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| Gene : | CD3E |
| Gene ID : | 916 |
| Uniprot ID : | P07766 |
| Alternative Name : | CD3E, CD3 epsilon, CD3 TCR complex, CD3E, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex |
| Amount : | 100 µg |
| Isotype : | Mouse IgG1, kappa |
| Immunogen Information : | Human T cell leukemia cells |
Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. CD3 is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. The CD3 complex is closely associated at the lymphocyte cell surface with T cell antigen receptor (TCR) and is involved in transducing antigen-recognition signals into cytoplasm of T cells and in regulating the cell surface expression of the TCR complex.
| Content : | 200 µg/ml of Ab Purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml. |
| Storage condition : | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. |
Flow Cytometry (1-2µg/million cells); ,Immunofluorescence (1-2µg/ml); ,Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
