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  7. Anti-CD45 / LCA (B-Cell Marker) Monoclonal Antibody(Clone: rPTPRC/1460)

Anti-CD45 / LCA (B-Cell Marker) Monoclonal Antibody(Clone: rPTPRC/1460)

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Fig. 1: Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Mouse Recombinant Monoclonal Antibody (rPTPRC/1460).

Anti-CD45 / LCA (B-Cell Marker) Monoclonal Antibody(Clone: rPTPRC/1460)
Anti-CD45 / LCA (B-Cell Marker) Monoclonal Antibody(Clone: rPTPRC/1460)
Anti-CD45 / LCA (B-Cell Marker) Monoclonal Antibody(Clone: rPTPRC/1460)
Anti-CD45 / LCA (B-Cell Marker) Monoclonal Antibody(Clone: rPTPRC/1460)

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Fig. 1: Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Mouse Recombinant Monoclonal Antibody (rPTPRC/1460).
Fig. 2: Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells using CD45 Mouse Recombinant Monoclonal Antibody (rPTPRC/1460) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).
Fig. 3: SDS-PAGE Analysis Purified CD45 Mouse Recombinant Monoclonal Antibody (rPTPRC/1460). Confirmation of Integrity and Purity of Antibody
Fig. 4: Western Blot Analysis of human spleen tissue lysates using CD45 Mouse Recombinant Monoclonal Antibody (rPTPRC/1460)

Product code: 36-3068

Clone name : rPTPRC/1460
Clonality : Monoclonal
Application : FACS, IF, WB, IHC
Reactivity : Human

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  •   20 µg

  •  100 µg

  • $199.00 

  • $459.00 

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  • Product Info
  • Antigen Details
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Amount : 100 µg
Isotype : Mouse IgG1, kappa
Content : 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Storage condition : Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
Gene : PTPRC
Gene ID : 5788
Uniprot ID : P08575
Alternative Name : B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein
Immunogen Information : Human recombinant full-length CD45 protein
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throµghout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.

Flow Cytometry (0.5-1µg/million cells); Immunofluorescence (0.5-1µg/ml); Western Blot (1-2µg/ml); ,Immunohistochemistry (Formalin-fixed) (0.5-1.0µg/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-CD45 / LCA (B-Cell Marker) Monoclonal Antibody(Clone: rPTPRC/1460)

Product code: 36-3068
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