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  7. Anti-H-Ras Monoclonal Antibody (Clone:H-RAS-03)

Anti-H-Ras Monoclonal Antibody (Clone:H-RAS-03)

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Figure 1: Western Blotting analysis (reducing conditions) of H-Ras in whole cell lysate using anti-H-Ras (H-RAS-03). . Lane 1: HeLa human cervix carcinoma cell line . Lane 2: K567 human leukemia cell line . Lane 3: RAJI human Burkitt lymphoma cell line

Anti-H-Ras Monoclonal Antibody (Clone:H-RAS-03)

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Product code: 30-1365

Clone name : H-RAS-03
Clonality : Monoclonal
Application : WB
Reactivity : Human

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0.1 mg
$356.00 

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Format : Purified
Amount : 0.1 mg
Isotype : Mouse IgG1
Purification : Purified by protein-A affinity chromatography
Storage condition : Store at 2-8°C. Do not freeze.
Gene : HRAS
Gene ID : 3265
Uniprot ID : P01112
Alternative Name : HRAS, HRAS1
Immunogen Information : Peptide corresponding to amino acids DIHQYREQIKRVKDSDDC of human H-Ras protein

H-Ras is one of three ubiquitously isoforms of Ras GTPase that operate at the intracellular leaflet of the plasma membrane to regulate multiple signal transduction pathways, such as mitogen-activated protein kinase (MAPK) cascade. H Ras is anchored to the plasma membrane by farnesyl and two palmityl residues. GTP loading decreases H-Ras affinity for lipid rafts and allows the protein to target to nonraft microdomains, the primary sites of H-Ras signaling. Sos protein and other guanine nucleotide-exchange factors catalyze dissociation of GDP from Ras. Besides its roles in the plasma membrane, active H-Ras also diffuses through the cytoplasm on nanoparticles termed rasosomes, which is dependent on Ras palmitoylation.

Western blotting: Recommended dilution: 1-2 μg/ml, reducing conditions.

 

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Nucleus, Cytoplasm, Cytoplasm
Post transnational modification: Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).
Tissue Specificity: Widely expressed.
BioGrid: 109501. 102 interactions.
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Anti-H-Ras Monoclonal Antibody (Clone:H-RAS-03)

Product code: 30-1365
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