Anti-H-Ras Monoclonal Antibody (Clone:H-RAS-03)
Figure 1: Western Blotting analysis (reducing conditions) of H-Ras in whole cell lysate using anti-H-Ras (H-RAS-03). . Lane 1: HeLa human cervix carcinoma cell line . Lane 2: K567 human leukemia cell line . Lane 3: RAJI human Burkitt lymphoma cell line
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| Format : | Purified |
| Amount : | 0.1 mg |
| Isotype : | Mouse IgG1 |
| Purification : | Purified by protein-A affinity chromatography |
| Storage condition : | Store at 2-8°C. Do not freeze. |
H-Ras is one of three ubiquitously isoforms of Ras GTPase that operate at the intracellular leaflet of the plasma membrane to regulate multiple signal transduction pathways, such as mitogen-activated protein kinase (MAPK) cascade. H Ras is anchored to the plasma membrane by farnesyl and two palmityl residues. GTP loading decreases H-Ras affinity for lipid rafts and allows the protein to target to nonraft microdomains, the primary sites of H-Ras signaling. Sos protein and other guanine nucleotide-exchange factors catalyze dissociation of GDP from Ras. Besides its roles in the plasma membrane, active H-Ras also diffuses through the cytoplasm on nanoparticles termed rasosomes, which is dependent on Ras palmitoylation.
Western blotting: Recommended dilution: 1-2 μg/ml, reducing conditions.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Nucleus, Cytoplasm, Cytoplasm |
| Post transnational modification: | Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). |
| Tissue Specificity: | Widely expressed. |
| BioGrid: | 109501. 102 interactions. |
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