Anti-H-Ras Monoclonal Antibody (Clone:H-RAS-03)
|Amount :||0.1 mg|
|Isotype :||Mouse IgG1|
|Purification :||Purified by protein-A affinity chromatography|
|Storage condition :||Store at 2-8°C. Do not freeze.|
H-Ras is one of three ubiquitously isoforms of Ras GTPase that operate at the intracellular leaflet of the plasma membrane to regulate multiple signal transduction pathways, such as mitogen-activated protein kinase (MAPK) cascade. H Ras is anchored to the plasma membrane by farnesyl and two palmityl residues. GTP loading decreases H-Ras affinity for lipid rafts and allows the protein to target to nonraft microdomains, the primary sites of H-Ras signaling. Sos protein and other guanine nucleotide-exchange factors catalyze dissociation of GDP from Ras. Besides its roles in the plasma membrane, active H-Ras also diffuses through the cytoplasm on nanoparticles termed rasosomes, which is dependent on Ras palmitoylation.
Western blotting: Recommended dilution: 1-2 μg/ml, reducing conditions.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|Subcellular location:||Nucleus, Cytoplasm, Cytoplasm|
|Post transnational modification:||Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).|
|Tissue Specificity:||Widely expressed.|
|BioGrid:||109501. 102 interactions.|