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  7. Anti-CD45RA (Leukocyte Marker) Monoclonal Antibody(Clone: K4B5)

Anti-CD45RA (Leukocyte Marker) Monoclonal Antibody(Clone: K4B5)

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Fig. 1: Formalin-fixed, paraffin-embedded human Spleen stained with CD45RA Mouse Monoclonal Antibody (K4B5).

Anti-CD45RA (Leukocyte Marker) Monoclonal Antibody(Clone: K4B5)
Anti-CD45RA (Leukocyte Marker) Monoclonal Antibody(Clone: K4B5)
Anti-CD45RA (Leukocyte Marker) Monoclonal Antibody(Clone: K4B5)
Anti-CD45RA (Leukocyte Marker) Monoclonal Antibody(Clone: K4B5)

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Fig. 1: Formalin-fixed, paraffin-embedded human Spleen stained with CD45RA Mouse Monoclonal Antibody (K4B5).
Fig. 2: Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RA Mouse Monoclonal Antibody (K4B5).
Fig. 3: Flow Cytometric Analysis of Jurkat cells using CD45RA Mouse Monoclonal Antibody (K4B5) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Fig. 4: Western Blot Analysis of human Spleen tissue lysates using CD45RA Mouse Monoclonal Antibody (K4B5)

Product code: 36-3067

Clone name : K4B5
Clonality : Monoclonal
Application : FACS, IF, WB, IHC
Reactivity : Human

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  •   20 µg

  •  100 µg

  • $199.00 

  • $459.00 

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  • Product Info
  • Antigen Details
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Amount : 100 µg
Isotype : Mouse IgG2a, kappa
Content : 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Storage condition : Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
Gene : PTPRC
Gene ID : 5788
Uniprot ID : P08575
Alternative Name : B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein
Immunogen Information : Stimulated human leukocytes
Recognizes a protein of 205kDa-220kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

Flow Cytometry (0.5-1µg/million cells); Immunofluorescence (1-2µg/ml); Western Blot (1-2µg/ml); Immunohistochemistry (Formalin-fixed) (0.5-1µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-CD45RA (Leukocyte Marker) Monoclonal Antibody(Clone: K4B5)

Product code: 36-3067
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