Anti-Cytokeratin 8/18 Monoclonal Antibody(Clone: SPM192 + SPM265)
Fig. 1: Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with Cytokeratin 8/18 Monoclonal Antibody (SPM192 + SPM265).
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| Amount : | 100 µg |
| Isotype : | Mouse IgG1, kappa + Mouse IgG1, kappa |
| Content : | 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage condition : | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. |
| Gene : | KRT8 & KRT18 |
| Gene ID : | 3856; 3875 |
| Uniprot ID : | P05787; P05783 |
| Alternative Name : | CARD2; CK8; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; KRT8; Type-II Keratin Kb8. Cell Proliferation-inducing Gene 46 Protein; CK18; CYK18Cytokeratin Endo B; K18; Keratin-18; Kerd; KRT18 |
| Immunogen Information : | Keratin preparation from a human carcinoma (SPM192); PMC-42 human breast carcinoma cells (SPM265) |
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
Flow Cytometry (0.5-1µg/million cells); Immunofluorescence (1-2µg/ml); ,Western Blot (0.5-1.0µg/ml); ,Immunohistochemistry (Formalin-fixed) (0.5-1.0µg/ml for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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