Anti-Spectrin Alpha 1 (Erythrocyte Marker) Monoclonal Antibody(Clone: SPTA1/1832)
Fig. 1: Formalin-fixed, paraffin-embedded human Placenta stained with Spectrin alpha 1 (SPTA1) Mouse Monoclonal Antibody (SPTA1/1832).
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| Amount : | 100 µg |
| Isotype : | Mouse IgG2b, kappa |
| Content : | 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage condition : | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. |
| Gene : | SPTA1 |
| Gene ID : | 6708 |
| Uniprot ID : | P02549 |
| Alternative Name : | Alpha I spectrin; Elliptocytosis 2 (EL2); Erythrocyte alpha spectrin; Erythroid alpha-spectrin; HPP; HS3; Spectrin alpha erythrocytic 1; SPH3; SPTA1 |
| Immunogen Information : | Recombinant human SPTA fragment (around aa356-475) (exact sequence is proprietary) |
Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. Spectrins function as membrane organizers and stabilizers, composed of non-homologous ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.
ELISA (Use Ab at 2-4µg/ml for coating) (Order Ab without BSA); Western Blot (0.5-2µg/ml). Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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