Fig. 1: Formalin-fixed, paraffin-embedded human Melanoma stained with gp100 Rabbit Polyclonal Antibody.
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Product code: 36-3158
| Clone name : | N/A |
| Application : | FACS, IF, IHC |
| Reactivity : | Human |
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| Gene : | SILV |
| Gene ID : | 6490 |
| Uniprot ID : | P40967 |
| Alternative Name : | 95kDa melanocyte-specific secreted glycoprotein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte protein Pmel 17, Melanoma associated ME20 antigen, Melanosomal matrix protein17, p100, p26, PMEL17, Premelanosome protein, Secreted melanoma-associated ME20 antigen, SILV, Silver homolog |
| Amount : | 100 µg |
| Isotype : | Rabbit IgG |
| Immunogen Information : | Recombinant full-length human SILV protein |
Cytotoxic T lymphocytes (CTL's) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein.
| Content : | 200 µg/ml of Ab Purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage condition : | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. |
Flow Cytometry (0.5-1µg/million cells); Immunofluorescence (0.5-1µg/ml); Immunohistochemistry (Formalin-fixed) (0.5-1.0µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
