Anti-GST / Glutathione-S-Transferase, GST tag Monoclonal Antibody (Clone:S-tag-05)
Figure 1: Isolation of Glutathione-S-Transferase (GST) overexpressed in E. coli.Western blot was immunostained by anti-GST (S-tag-05). Lane 1: affinity purification of GST from bacterial cell lysate using commercial sorbent Glutathione-Sepharose. Lane 2: affinity purification of GST from bacterial cell lysate using immunosorbent prepared from anti-GST (S-tag-02; )
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|Amount :||0.1 mg|
|Isotype :||Mouse IgG2b|
|Immunogen Information :||GST fusion protein|
The GST (Glutathione-S-Transferase) is a 26kDa protein encoded by the parasite Schistosoma japonicum, easily purified by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors. Antibodies against GST will detect the expressed GST protein or a GST-fusion protein, and can be utilized for immuno-purification of GST-fusion proteins.
|Purification :||Purified by protein-A affinity chromatography|
|Storage condition :||Store at 2-8°C. Do not freeze.|
Western Blotting Recommended dilution: 1 µg/ml
Positive control: GST fusion protein
For Research Use Only. Not for use in diagnostic/therapeutics procedures.