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  7. Anti-Human CD274 FITC (Clone : 29E.2A3)

Anti-Human CD274 FITC (Clone : 29E.2A3)

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Figure 1 : Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cell suspension stained using anti-human CD274 (29E.2A3) FITC antibody (4 µl reagent per milion cells in 100 µl of cell suspension).

Anti-Human CD274 FITC (Clone : 29E.2A3)
Anti-Human CD274 FITC (Clone : 29E.2A3)

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Figure 1 : Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cell suspension stained using anti-human CD274 (29E.2A3) FITC antibody (4 µl reagent per milion cells in 100 µl of cell suspension).
Figure 2 : Separation of human CD274 positive cells (red-filled) from cellular debris (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cell suspension stained using anti-human CD274 (29E.2A3) FITC antibody (4 µl reagent per milion cells in 100 µl of cell suspension).

Product code: 30-2601

Clone name : 29E.2A3
Clonality : Monoclonal
Application : FACS
Reactivity : Human
Conjugate : FITC

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100 tests
$408.00 

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  • Product Info
  • Antigen Details
  • Description
  • Application Note
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Amount : 100 tests
Isotype : Mouse IgG2b kappa
Purification : The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Content : Formulation : Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage condition : Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light.
Gene : CD274
Gene ID : 29126
Alternative Name : B7H1, PDL1, PDCD1L1, PDCD1LG1, PDCD1 ligand 1, B7-H1,CD274 molecule
Immunogen Information : Full length human CD274
CD274 / PD-L1 (programmed death ligand-1), also known as B7-H1, is a member of the B7 family of regulatory proteins. It can act as both costimulatory and coinhibitory molecule for T cells. Interaction with its ligand CD279 (PD1) appears to be important in the maintenance of peripheral tolerance and in prevention of tumor rejection. Even pathogens (e.g. Schistosoma) may exploit CD274 to evade an immune response. Besides CD279, existence of other receptor(s) for CD274 is likely.
Specificity : The mouse monoclonal antibody 29E.2A3 recognizes an extracellular epitope of CD274 / PD-L1 (also known as B7-H1), a 40 kDa type I transmembrane protein expressed by dendritic cells, activated T cells, activated monocytes, and in various tissues, above all in heart and skeletal muscle, placenta and lung, and in many cancer cells, including T cell lymphomas, melanomas, and glioblastomas.

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-Human CD274 FITC (Clone : 29E.2A3)

Product code: 30-2601
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