Anti-Human CD77 Antibody (Clone : 38.13)
Figure 1 : Separation of RAJI cells stained anti-human CD77 (38.13) Purified antibody (concentration in sample 1,67 µg/ml, GAR Alexa Fluor® 647, red-filled) from RAJI cells unstained by primary antibody (GAR Alexa Fluor® 647, black-dashed) in flow cytometry analysis (surface staining) of RAJI cell suspension.
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| Format : | Purified |
| Amount : | 0.1 mg |
| Isotype : | Lewis Rat IgM |
| Purification : | Purified by protein-A affinity chromatography |
| Content : | 1 mg/ml Formulation : Phosphate buffered saline (PBS) solution with 15 mM sodium azide |
| Storage condition : | Store at 2-8°C. Do not freeze. |
| Alternative Name : | globotriaosylceramide Gb3, Gal-alpha1-4Gal-beta1-4Glc-beta1-Cer, P blood group |
| Immunogen Information : | Daudi cell line (Burkitt's lymphoma) |
CD77 (globotriaosylceramide Gb3), also known as the Pk blood group antigen, BLA (Burkitt´s lymphoma associated antigen), or CTH (ceramide trihexoside) is a neutral glycosphingolipid composed of three carbohydrate molecules linked to a lipid moiety in the cell membrane (Gal-alpha1-4Gal-beta1-4Glc-beta1-Cer). It is expressed on germinal center B cells, Burkitt´s lymphoma cells, it can be induced on extrafolicular B cells and it is also found on endothelia and epithelia. CD77 may be involved in elimination of germinal center B cells that fail to produce high affinity antibodies, and serves also as receptor for shiga toxin and verotoxin.
Specificity : The rat monoclonal antibody 38.13 recognizes CD77 (globotriaosylceramide Gb3), a neutral glycosphingolipid expressed mainly on the surface of B cell populations, such as germinal center B cells and Burkitt´s lymphoma cells.
Specificity : The rat monoclonal antibody 38.13 recognizes CD77 (globotriaosylceramide Gb3), a neutral glycosphingolipid expressed mainly on the surface of B cell populations, such as germinal center B cells and Burkitt´s lymphoma cells.
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