Monoclonal Antibody to mouse TIM-4
|Amount :||500 µg|
|Isotype :||Armenian hamster IgG|
|Content :||0.5 mg, 0.2 µm filtered antibody solution in PBS|
|Storage condition :||Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.|
The monoclonal antibody Kat5-18 recognizes T-cell immunoglobulin and mucin domain containing molecule (TIMD-4, also called TIM-4). TIMD-4 is a type I transmembrane protein belonging to the immunoglobulin superfamily, containing one Ig-like V-type (immunoglobulin-like) domain. TIMD-4 is expressed in Mac1+ cells in spleen, lymph nodes and fetal liver. TIMD-4 binds apoptotic cells by recognizing phosphatidylserine via its immunoglobulin domain. TIMD-4 in fibroblasts enhanced their ability to engulf apoptotic cells. TIMD-4 is involved in regulating T-cell proliferation and lymphotoxin signaling. TIMD-4 and TIMD-1 are phosphatidylserine receptors for the engulfment of apoptotic cells, and are also involved in intracellular signaling in which exosomes are involved. The monoclonal antibody Kat5-18 blocks the engulfment of apoptotic cells by thymic macrophages.
For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|Tissue Specificity:||Predominantly expressed in lymphoid tissues, such as spleen, lymph nodes, and Peyer patches. Also expressed in fetal liver, salivary gland, and spleen stromal cells, predominantly in the marginal zone and to a lesser extent throughout the white pulp. Not expressed by bone marrow-derived cells. In spleen, expressed mostly by stromal cells in T- and B-cell areas, but not by T or B-lymphocytes.|