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  7. Anti-Lewis A (Blood Group Antigen) Monoclonal Antibody(Clone: 7LE)

Anti-Lewis A (Blood Group Antigen) Monoclonal Antibody(Clone: 7LE)

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Fig. 1: Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Lewis A Monoclonal Antibody (7LE).

Anti-Lewis A (Blood Group Antigen) Monoclonal Antibody(Clone: 7LE)
Anti-Lewis A (Blood Group Antigen) Monoclonal Antibody(Clone: 7LE)

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Fig. 1: Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Lewis A Monoclonal Antibody (7LE).
Fig. 2: SDS-PAGE Analysis Purified Lewis A Monoclonal Antibody (7LE). Confirmation of Purity and Integrity of Antibody.

Product code: 36-3719

Clone name : 7LE
Clonality : Monoclonal
Application : FACS, IF, IHC
Reactivity : Human, Mouse

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  •   20 µg

  •  100 µg

  • $199.00 

  • $459.00 

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  • Product Info
  • Antigen Details
  • Description
  • Application Note
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Amount : 100 µg
Isotype : Mouse IgG1, kappa
Content : 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Storage condition : Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
Alternative Name : Lewis A antigen
Immunogen Information : Mucins isolated from ovarian cyst fluid
Recognizes a carbohydrate determinant of Gal 1-3(Fuc 1-4) GlcNAc which is blood group antigen Lewis A. It is present primarily on epithelial cells such as colon and kidneys. In the tumors and dedifferentiated tissues, decrease of Lewis A antigen was observed. Lewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

Flow Cytometry (0.5-1µg/million cells); Immunofluorescence (0.5-1µg/ml); Immunohistochemistry (Formalin-fixed) (0.5-1.0µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-Lewis A (Blood Group Antigen) Monoclonal Antibody(Clone: 7LE)

Product code: 36-3719
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