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  7. Anti-Microphthalmia Transcription Factor (MITF) Monoclonal Antibody(Clone: MITF/2987R)

Anti-Microphthalmia Transcription Factor (MITF) Monoclonal Antibody(Clone: MITF/2987R)

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Fig. 1: Formalin-fixed, paraffin-embedded human Melanoma stained with MITF Recombinant Rabbit Monoclonal Antibody (MITF/2987R).

Anti-Microphthalmia Transcription Factor (MITF) Monoclonal Antibody(Clone: MITF/2987R)
Anti-Microphthalmia Transcription Factor (MITF) Monoclonal Antibody(Clone: MITF/2987R)
Anti-Microphthalmia Transcription Factor (MITF) Monoclonal Antibody(Clone: MITF/2987R)

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Fig. 1: Formalin-fixed, paraffin-embedded human Melanoma stained with MITF Recombinant Rabbit Monoclonal Antibody (MITF/2987R).
Fig. 2: Formalin-fixed, paraffin-embedded human Melanoma stained with MITF Recombinant Rabbit Monoclonal Antibody (MITF/2987R).
Fig. 3: SDS-PAGE Analysis Purified MITF Recombinant Rabbit Monoclonal Antibody (MITF/2987R). Confirmation of Purity and Integrity of Antibody.

Product code: 36-2770

Clone name : MITF/2987R
Clonality : Monoclonal
Application : FACS, IF, IHC
Reactivity : Human

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  •   20 µg

  •  100 µg

  • $199.00 

  • $459.00 

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  • Product Info
  • Antigen Details
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Amount : 100 µg
Isotype : Rabbit IgG
Content : 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Storage condition : Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
Gene : MITF
Gene ID : 4286
Uniprot ID : O75030
Alternative Name : BHLHE32; Class E basic helix-loop-helix protein 32 (bHLHe32); CMM8; Mi; Microphthalmia-associated transcription factor; MITF; WS2; WS2A
Immunogen Information : Recombinant full-length human MiTF protein
MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. This MAb recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.

Flow Cytometry (0.5-1µg/million cells); Immunofluorescence (0.5-1µg/ml); Immunohistochemistry (Formalin-fixed) (0.5-1.0µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-Microphthalmia Transcription Factor (MITF) Monoclonal Antibody(Clone: MITF/2987R)

Product code: 36-2770
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