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  7. Anti-MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Monoclonal Antibody(Clone: SPM493)

Anti-MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Monoclonal Antibody(Clone: SPM493)

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Fig. 1: Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC1 / CA15-3 / EMA Mouse Monoclonal Antibody (SPM493).

Anti-MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Monoclonal Antibody(Clone: SPM493)
Anti-MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Monoclonal Antibody(Clone: SPM493)

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Fig. 1: Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC1 / CA15-3 / EMA Mouse Monoclonal Antibody (SPM493).
Fig. 2: Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (HMPV) followed by Goat anti-Mouse IgG-CF488 (Green). Nuclear counterstain is RedDot'

Product code: 36-2811

Clone name : SPM493
Clonality : Monoclonal
Application : IF
Reactivity : Human

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  •   20 µg

  •  100 µg

  • $199.00 

  • $459.00 

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  • Product Info
  • Antigen Details
  • Description
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Amount : 100 µg
Isotype : Mouse IgG1, kappa
Content : 200 µg/ml of Ab Purified from Bioreactor Concentrate on Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Storage condition : Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
Gene : MUC1
Gene ID : 4582
Uniprot ID : P15941
Alternative Name : Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, Epithelial Membrane Antigen, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen
Immunogen Information : Human breast cancer cell line ZR-75 cells

This MAb reacts with MUC1/EMA. It is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells. It is expressed on most secretory epithelium, including mammary gland. It is expressed abundantly in lactating mammary glands and over expressed abundantly in 90% breast carcinomas and metastases.MUC1/EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack.In immunohistochemical assays, it superbly stains routine formalin-paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Immunofluorescence (1-2µg/ml); Immunohistochemistry (Formalin-fixed) (0.25-0.5µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Monoclonal Antibody(Clone: SPM493)

Product code: 36-2811
*specify in mg or ml

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