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  7. Anti-p53 Tumor Suppressor Protein Monoclonal Antibody(Clone: rTP53/1739)

Anti-p53 Tumor Suppressor Protein Monoclonal Antibody(Clone: rTP53/1739)

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Fig. 1: Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with p53 Mouse Recombinant Monoclonal Antibody (rTP53/1739)

Anti-p53 Tumor Suppressor Protein Monoclonal Antibody(Clone: rTP53/1739)
Anti-p53 Tumor Suppressor Protein Monoclonal Antibody(Clone: rTP53/1739)
Anti-p53 Tumor Suppressor Protein Monoclonal Antibody(Clone: rTP53/1739)
Anti-p53 Tumor Suppressor Protein Monoclonal Antibody(Clone: rTP53/1739)

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Fig. 1: Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with p53 Mouse Recombinant Monoclonal Antibody (rTP53/1739)
Fig. 2: SDS-PAGE Analysis Purified p53 Mouse Recombinant Monoclonal Antibody (rTP53/1739). Confirmation of Purity and Integrity of Antibody.
Fig. 3: Western Blot Analysis of HeLa cell lysate using p53 Mouse Recombinant Monoclonal Antibody (rTP53/1739).
Fig. 4: Analysis of Protein Array containing more than 19,000 full-length human proteins using p53 Mouse Monoclonal Antibody (rTP53/1739). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Product code: 36-3291

Clone name : rTP53/1739
Clonality : Monoclonal
Application : WB, IHC
Reactivity : Human

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  •  100 µg

  • $199.00 

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  • Antigen Details
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Amount : 100 µg
Isotype : Mouse IgG1, kappa
Content : 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Storage condition : Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
Gene : TP53
Gene ID : 7157
Uniprot ID : P04637
Alternative Name : Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53
Immunogen Information : Recombinant full-length human TP53 protein
The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. It is a tumor suppressor protein expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast, lung, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in many malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

Western Blot (1-2µg/ml); ,Immunohistochemistry (Formalin-fixed) (0.5-1.0µg/ml for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-p53 Tumor Suppressor Protein Monoclonal Antibody(Clone: rTP53/1739)

Product code: 36-3291
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