Anti-PGP9.5 / UchL1 (pan-Neuronal Marker) Monoclonal Antibody(Clone: rUCHL1/775)

Fig. 1: Formalin-fixed, paraffin-embedded human Cerebellum stained with Pgp9.5 Mouse Recombinant Monoclonal Antibody (rUCHL1/775).

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Fig. 1: Formalin-fixed, paraffin-embedded human Cerebellum stained with Pgp9.5 Mouse Recombinant Monoclonal Antibody (rUCHL1/775).

Fig. 2: SDS-PAGE Analysis Purified Pgp9.5 Mouse Recombinant Monoclonal Antibody (rUCHL1/775). Confirmation of Purity and Integrity of Antibody.

Fig. 3: Western Blot Analysis of human brain tissue lysate using Pgp9.5 Mouse Recombinant Monoclonal Antibody (rUCHL1/775).

Fig. 4: Formalin-fixed, paraffin-embedded human Pancreas stained with Pgp9.5 Mouse Recombinant Monoclonal Antibody (rUCHL1/775).

Fig. 5: Flow Cytometric Analysis of T98G cells using Pgp9.5 Mouse Recombinant MAb (rUCHL1/775) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Fig. 6: Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with Pgp9.5 Mouse Recombinant MAb (rUCHL1/775) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red).

Fig. 7: Analysis of Protein Array containing >19,000 full-length human proteins using Pgp9.5 Mouse Recombinant Monoclonal Antibody (rUCHL1/775) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Product code: 36-3345

Clone name :	rUCHL1/775
Application :	WB, FACS, IF, IHC
Reactivity :	Human, Rat

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Download TDS MSDS

Shipping Info:

For estimated delivery dates, please contact us at support@abeomics.com

Download TDS MSDS


Gene :	UCHL1
Gene ID :	7345
Uniprot ID :	P09936
Alternative Name :	Gracile Axonal Dystrophy; Neuron Cytoplasmic Protein 9.5; Park5; Parkinson Disease 5; PGP95; Protein Gene Product 9.5; Ubiquitin Carboxyl-terminal Esterase L1; Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1; Ubiquitin Thioesterase L1; Ubiquitin Thiolesterase L1
Amount :	100 µg
Isotype :	Mouse IgG1, kappa
Immunogen Information :	Recombinant full-length human UCHL1 protein

This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.

Content :	200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Storage condition :	Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Western Blot (1-2µg/ml); Flow Cytometry (1-2µg/million cells); Immunofluorescence (1-2µg/ml); Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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