Anti-Rat CD8a Low Endotoxin Antibody (Clone : OX-8)
|Format :||Low Endotoxin|
|Amount :||0.1 mg|
|Isotype :||Mouse IgG1 kappa|
|Purification :||Purified by protein-A affinity chromatography|
|Content :||1 mg/ml
Formulation : Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
|Storage condition :||Store at 2-8°C. Do not freeze.|
|Gene ID :||24930|
|Alternative Name :||Ly-2, Ly-B, Ly-35, Lyt-2,CD8a molecule|
|Immunogen Information :||High Mw glycoproteins from rat thymocytes|
The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Specificity : The mouse monoclonal antibody OX-8 recognizes the hinge-like membrane-proximal extracellular domain of rat CD8a (32-34 kDa; alpha chain of the CD8 antigen).