Anti-Spectrin beta III (SPTBN2) Monoclonal Antibody(Clone: SPTBN2/2894R)
Fig. 1: Western Blot Analysis of HeLa cell lysates using Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R).
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| Amount : | 100 µg |
| Isotype : | Rabbit IgG |
| Content : | 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage condition : | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. |
| Gene : | SPTBN2 |
| Gene ID : | 6712 |
| Uniprot ID : | O15020 |
| Alternative Name : | Beta III spectrin; SCA5; Spectrin beta chain brain 2; Spectrin beta non-erythrocytic 2; Spectrin non-erythroid beta chain 2; Spinocerebellar ataxia 5; SPTBN2 |
| Immunogen Information : | Recombinant human SPTBN2 fragment (aa356-475) (exact sequence is proprietary) |
Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II) four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.
Flow Cytometry (1-2µg/million cells); (ELISA (Use Ab at 2-4µg/ml for coating) (Order Ab without BSA);Western Blot (0.5-1.0µg/ml);Immunohistochemistry (Formalin-fixed) (0.5-1.0µg/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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